Blade of the Immortal

Rin and Manji meet with Shira and Hyakurin at the Mugai-ryuu to discuss plans to defeat the Ittou-ryuu, Meanwhile, Magatsu returns days later to find O-Ren dead. Hyakurin learns through Makoto, her spy at the Ittou-ryuu, that Anotsu is dressing as a geisha to enter the Kobotoke gate. They keep watch and Rin helps them identify him, but after Shira and Rin follow the figure for some distance, they realize that they have been duped and led into a trap by a hired prostitute. They are attacked by the Ittou-ryuu, Saikaya and Sei disguised as pharmacists, however Shira kills them both and begins to slash and rape the woman. Rin draws her sword, but as Shira disarms Rin, Manji arrives and severs Shira's hand and shocked, he runs off. Manji's act is witnessed by locals, and he suspects they will be blamed for the bloodbath and dead pharmacists. (Source: Wikipedia)